Human Transthyretin (TTR) ELISA Kit

Details: Overview Reactivity: Human Methode Type: Sandwich ELISA Detection Quantity: 96 tests Sample type： serum, plasma, Urine,tissue homogenates, cell culture supernates Detection range : 0.3ng/ml-10 ng/ml Kit Components: Assay plate (12 × 8 coated Microwells) ……………………..1 Standard: 13.5 ng/ml………………………………………… 1×0.5ml Standard Diluent…………………………………………… 1×1.5ml HRP-Conjugate Reagent …………………………………… 1×6ml Sample Diluent……………………………………………… 1×6ml Chromogen Solution A…………………………………………1×6ml Chromogen Solution B…………………………………………1×6ml Stop Solution …………………………………………………1×6ml Wash Solution…………………………………………………1×20ml×30 fold User manual……………………………………………………1 Adhesive Strip……………………………………………………2 Product Principle: The kit is for the quantitative level of TTR in the sample, adopt purified Human TTR antibody to coat microtiter plate, make solid-phase antibody, then add TTR to wells, Combine TTR antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of TTR in the samples is then determined by comparing the O.D. of the samples to the standard curve.